Background: The ever increasing competition among organizations has led them looking for ways to gain success. Organizational learning and quality of working life are considered as the basic tools for gaining organizational competitive advantages. This study aimed to determine the effect of quality of working life on organizational learning among nurses working in the teaching hospitals affiliated to Shiraz University of Medical Sciences in 2016. Methods: This was a cross-sectional descriptive-analytical study. A sample size of 185 nurses was selected through randomized stratified sampling. The required data were collected using two questionnaires assessing organizational learning and quality of working life. The collected data were analyzed through SPSS23 and using independent t-test, ANOVA, Pearson correlation, and multiple linear regression. Results: Organizational learning and quality of working life were at moderate level in the studied nurses. Moreover, there was a significant positive correlation between organizational learning and quality of working life (r=0.66, P<0.001). Also, the results of multiple linear regression analysis showed that "participation in decision-making","security","career advancements", and "nurses' motivation for doing work and tasks" had significant and positive effects on organizational learning (P<0.05). Conclusion: According to the results, hospital managers can increase nurses' quality of working life and accordingly, organizational learning through involving nurses in the related decision-making processes and providing opportunities for their creativity, learning and experiencing in line of their career advancement.